The Small Intestine in the Acute Period of Spinal Injury: Early Metabolic Disorders According to Fluorescence-Lifetime Imaging FLIM

RELEVANCE A special place in the development of enteral insufficiency is given to dysproteinemia, which one leading causes decubital ulcers patients with spinal cord injury. Early nutrition partially solved this problem, but incidence bedsores still remains high and reaches 68%. The risk metabolic disorders acute period injury largely determined by non-occlusive intestinal ischemia against background shock, neurohumoral dysregulation; intra-intestinal intra-abdominal hypertension; change microflora. Pathological changes wall occur during first 20 days after further exacerbate chronic maldigestion, malabsorption, dyskinesia traumatic disease. New knowledge about features early disease will reduce decubitus ulcerative defects. AIM OF THE STUDY To study dynamics processes tissues small intestine MATERIAL AND METHODS Wistar rats (n=22). Spinal was simulated complete transection at level Th5–Th6 vertebrae. assessment cells serous membrane performed immediately, 3 24 hours metabolism assessed vivo using fluorescence time-resolved macroimaging technology FLIM autofluorescence spectral channel cofactor nicotinamide adenine dinucleotide (phosphate). RESULTS accompanied a endogenous intestine: statistically significant decrease mean lifetime (τm), long component (τ2), relative contribution (а2) h recorded. observed confirm catabolic type CONCLUSION For time experiment it has been shown that violation intestine. This fact requires more balanced approach calculating calorie content nutrients used for